Self-assembled films of dendrimers and metallophthalocyanines as FET-based glucose biosensors.
Identifieur interne : 001149 ( Main/Exploration ); précédent : 001148; suivant : 001150Self-assembled films of dendrimers and metallophthalocyanines as FET-based glucose biosensors.
Auteurs : RBID : pubmed:22163704English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Glucose.
- chemical , chemistry : Aziridines, Dendrimers, Indoles, Nickel.
- enzymology : Aspergillus niger.
- instrumentation : Biosensing Techniques.
- chemical , metabolism : Glucose Oxidase.
- chemical : Buffers, Hydrogen-Ion Concentration, Recycling, Transistors, Electronic.
Abstract
Separative extended gate field effect transistor (SEGFET) type devices have been used as an ion sensor or biosensor as an alternative to traditional ion sensitive field effect transistors (ISFETs) due to their robustness, ease of fabrication, low cost and possibility of FET isolation from the chemical environment. The layer-by-layer technique allows the combination of different materials with suitable properties for enzyme immobilization on simple platforms such as the extended gate of SEGFET devices enabling the fabrication of biosensors. Here, glucose biosensors based on dendrimers and metallophthalocyanines (MPcs) in the form of layer-by-layer (LbL) films, assembled on indium tin oxide (ITO) as separative extended gate material, has been produced. NH(3)(+) groups in the dendrimer allow electrostatic interactions or covalent bonds with the enzyme (glucose oxidase). Relevant parameters such as optimum pH, buffer concentration and presence of serum bovine albumin (BSA) in the immobilization process were analyzed. The relationship between the output voltage and glucose concentration shows that upon detection of a specific analyte, the sub-products of the enzymatic reaction change the pH locally, affecting the output signal of the FET transducer. In addition, dendritic layers offer a nanoporous environment, which may be permeable to H(+) ions, improving the sensibility as modified electrodes for glucose biosensing.
DOI: 10.3390/s111009442
PubMed: 22163704
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Self-assembled films of dendrimers and metallophthalocyanines as FET-based glucose biosensors.</title><author><name sortKey="Vieira, Nirton C S" uniqKey="Vieira N">Nirton C S Vieira</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Física de São Carlos, Universidade de São Paulo, CP 369, São Carlos, SP 13560-970, Brazil. nirton@ursa.ifsc.usp.br</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Instituto de Física de São Carlos, Universidade de São Paulo, CP 369, São Carlos, SP 13560-970</wicri:regionArea></affiliation></author><author><name sortKey="Figueiredo, Alessandra" uniqKey="Figueiredo A">Alessandra Figueiredo</name></author><author><name sortKey="De Queiroz, Alvaro A A" uniqKey="De Queiroz A">Alvaro A A de Queiroz</name></author><author><name sortKey="Zucolotto, Valtencir" uniqKey="Zucolotto V">Valtencir Zucolotto</name></author><author><name sortKey="Guimar Es, Francisco E G" uniqKey="Guimar Es F">Francisco E G Guimarães</name></author></titleStmt><publicationStmt><date when="2011">2011</date><idno type="doi">10.3390/s111009442</idno><idno type="RBID">pubmed:22163704</idno><idno type="pmid">22163704</idno><idno type="wicri:Area/Main/Corpus">001011</idno><idno type="wicri:Area/Main/Curation">001011</idno><idno type="wicri:Area/Main/Exploration">001149</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aspergillus niger (enzymology)</term><term>Aziridines (chemistry)</term><term>Biosensing Techniques (instrumentation)</term><term>Buffers</term><term>Dendrimers (chemistry)</term><term>Glucose (analysis)</term><term>Glucose Oxidase (metabolism)</term><term>Hydrogen-Ion Concentration</term><term>Indoles (chemistry)</term><term>Nickel (chemistry)</term><term>Recycling</term><term>Transistors, Electronic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Glucose</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Aziridines</term><term>Dendrimers</term><term>Indoles</term><term>Nickel</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Aspergillus niger</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucose Oxidase</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Buffers</term><term>Hydrogen-Ion Concentration</term><term>Recycling</term><term>Transistors, Electronic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Separative extended gate field effect transistor (SEGFET) type devices have been used as an ion sensor or biosensor as an alternative to traditional ion sensitive field effect transistors (ISFETs) due to their robustness, ease of fabrication, low cost and possibility of FET isolation from the chemical environment. The layer-by-layer technique allows the combination of different materials with suitable properties for enzyme immobilization on simple platforms such as the extended gate of SEGFET devices enabling the fabrication of biosensors. Here, glucose biosensors based on dendrimers and metallophthalocyanines (MPcs) in the form of layer-by-layer (LbL) films, assembled on indium tin oxide (ITO) as separative extended gate material, has been produced. NH(3)(+) groups in the dendrimer allow electrostatic interactions or covalent bonds with the enzyme (glucose oxidase). Relevant parameters such as optimum pH, buffer concentration and presence of serum bovine albumin (BSA) in the immobilization process were analyzed. The relationship between the output voltage and glucose concentration shows that upon detection of a specific analyte, the sub-products of the enzymatic reaction change the pH locally, affecting the output signal of the FET transducer. In addition, dendritic layers offer a nanoporous environment, which may be permeable to H(+) ions, improving the sensibility as modified electrodes for glucose biosensing.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">22163704</PMID><DateCreated><Year>2012</Year><Month>02</Month><Day>07</Day></DateCreated><DateCompleted><Year>2012</Year><Month>06</Month><Day>05</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1424-8220</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>10</Issue><PubDate><Year>2011</Year></PubDate></JournalIssue><Title>Sensors (Basel, Switzerland)</Title><ISOAbbreviation>Sensors (Basel)</ISOAbbreviation></Journal><ArticleTitle>Self-assembled films of dendrimers and metallophthalocyanines as FET-based glucose biosensors.</ArticleTitle><Pagination><MedlinePgn>9442-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3390/s111009442</ELocationID><Abstract><AbstractText>Separative extended gate field effect transistor (SEGFET) type devices have been used as an ion sensor or biosensor as an alternative to traditional ion sensitive field effect transistors (ISFETs) due to their robustness, ease of fabrication, low cost and possibility of FET isolation from the chemical environment. The layer-by-layer technique allows the combination of different materials with suitable properties for enzyme immobilization on simple platforms such as the extended gate of SEGFET devices enabling the fabrication of biosensors. Here, glucose biosensors based on dendrimers and metallophthalocyanines (MPcs) in the form of layer-by-layer (LbL) films, assembled on indium tin oxide (ITO) as separative extended gate material, has been produced. NH(3)(+) groups in the dendrimer allow electrostatic interactions or covalent bonds with the enzyme (glucose oxidase). Relevant parameters such as optimum pH, buffer concentration and presence of serum bovine albumin (BSA) in the immobilization process were analyzed. The relationship between the output voltage and glucose concentration shows that upon detection of a specific analyte, the sub-products of the enzymatic reaction change the pH locally, affecting the output signal of the FET transducer. In addition, dendritic layers offer a nanoporous environment, which may be permeable to H(+) ions, improving the sensibility as modified electrodes for glucose biosensing.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vieira</LastName><ForeName>Nirton C S</ForeName><Initials>NC</Initials><Affiliation>Instituto de Física de São Carlos, Universidade de São Paulo, CP 369, São Carlos, SP 13560-970, Brazil. nirton@ursa.ifsc.usp.br</Affiliation></Author><Author ValidYN="Y"><LastName>Figueiredo</LastName><ForeName>Alessandra</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>de Queiroz</LastName><ForeName>Alvaro A A</ForeName><Initials>AA</Initials></Author><Author ValidYN="Y"><LastName>Zucolotto</LastName><ForeName>Valtencir</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Guimarães</LastName><ForeName>Francisco E G</ForeName><Initials>FE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>10</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Sensors (Basel)</MedlineTA><NlmUniqueID>101204366</NlmUniqueID><ISSNLinking>1424-8220</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Aziridines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Buffers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Dendrimers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Indoles</NameOfSubstance></Chemical><Chemical><RegistryNumber>574-93-6</RegistryNumber><NameOfSubstance>phthalocyanine</NameOfSubstance></Chemical><Chemical><RegistryNumber>75-55-8</RegistryNumber><NameOfSubstance>propyleneimine</NameOfSubstance></Chemical><Chemical><RegistryNumber>7OV03QG267</RegistryNumber><NameOfSubstance>Nickel</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.3.4</RegistryNumber><NameOfSubstance>Glucose Oxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance>Glucose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Biosens Bioelectron. 2001 Jan;16(1-2):121-31</RefSource><PMID Version="1">11261847</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Anal Bioanal Chem. 2003 Oct;377(3):496-506</RefSource><PMID Version="1">12904953</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biosens Bioelectron. 2004 May 15;19(10):1295-300</RefSource><PMID Version="1">15046762</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Anal Chem. 1985 Aug;57(9):1920-3</RefSource><PMID Version="1">4037345</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biosens Bioelectron. 2010 Feb 15;25(6):1254-63</RefSource><PMID Version="1">19889526</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Anal Chem. 1994 Jan 15;66(2):205-10</RefSource><PMID Version="1">8116877</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Am Chem Soc. 2008 Mar 26;130(12):3712-3</RefSource><PMID Version="1">18321103</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Phys Chem Chem Phys. 2009 Jul 7;11(25):5086-91</RefSource><PMID Version="1">19562139</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biosens Bioelectron. 2010 Sep 15;26(1):275-8</RefSource><PMID Version="1">20598519</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biosensors. 1987-1988;3(3):161-86</RefSource><PMID Version="1">3447596</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Aspergillus niger</DescriptorName><QualifierName MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Aziridines</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Biosensing Techniques</DescriptorName><QualifierName MajorTopicYN="Y">instrumentation</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Buffers</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Dendrimers</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Glucose</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Glucose Oxidase</DescriptorName><QualifierName MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indoles</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nickel</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Recycling</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Transistors, Electronic</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC3231248</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">enzyme immobilization</Keyword><Keyword MajorTopicYN="N">field effect transistor</Keyword><Keyword MajorTopicYN="N">glucose biosensor</Keyword><Keyword MajorTopicYN="N">layer by layer</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>8</Month><Day>31</Day></PubMedPubDate><PubMedPubDate 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